IGEVET   21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
A new molecular screening tool for the detection of chromosomal abnormalities in donkeys
Autor/es:
POYATO-BONILLA, JULIA; VALERA, MERCEDES; MOLINA, ANTONIO; DEMYDA-PEYRÁS, SEBASTIÁN; DORADO, JESÚS; ANAYA-CALVO, GABRIEL; MORENO-MILLÁN, MIGUEL
Revista:
REPRODUCTION IN DOMESTIC ANIMALS (1990)
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2019
ISSN:
0936-6768
Resumen:
Chromosomal abnormalities are a major cause of infertility and reproductive prob‐lems in equids. Nowadays, their detection is rising due to the use of new diagnostic tools based on molecular markers instead of karyotyping. Reports of this kind of ge‐netic aberrations in domestic donkeys (Equus asinus) are extremely scarce, despite their importance in human activities. In the present study, we analysed the imple‐mentation of a short‐tandem‐repeat (STR)‐based molecular method initially devel‐oped for horses, as a diagnostic tool to detect chromosomal abnormalities in donkeys. The frequency of five X‐linked (LEX003, LEX026, TKY38, TK Y270 and UCEDQ502) and one Y‐linked (ECAYM2) molecular markers and one Y‐linked gene (sex‐determining region Y, SRY) was characterized in 121 donkeys from two diverse breeds, the Spanish Andalusian and the African Moroccan breeds. The molecular panel showed 100% sensitivity and 99.67% specificity in detecting 10 different chromosomal abnormali‐ties in the species. In conclusion, this methodology is a valid, rapid and low‐cost tool for the detection and characterization of chromosomal abnormalities in domestic donkeys.