Isolation of Shiga toxin-producing Escherichia coli from ground beef using multiple combinations of enrichment broths and selective agars

GALLI LUCÍA; PADOLA N L; PIÑEYRO P; ORTEGA EMANUEL; BRUSA V.; LINARES LUCIANO HORACIO; LEOTTA G.A.

FOODBORNE PATHOGENS AND DISEASE

MARY ANN LIEBERT INC

Lugar: New York; Año: 2016 vol. 13 p. 163 - 170

1535-3141

Shiga toxin-producing Escherichia coli (STEC) are food-borne pathogens, and beef cattle are recognized as the principal reservoir. The aims of this study were: 1. to identify the most sensitive combination of selective enrichment broths and agars for STEC isolation in artificially inoculated ground beef samples, and 2. to evaluate the most efficient combination(s) of methods for naturally contaminated ground beef samples. A total of 192 ground beef samples were artificially inoculated with STEC and non-stx bacterial strains. A combination of four enrichment broths and three agars were evaluated for sensitivity, specificity, and positive predictive value for STEC isolation from experimentally inoculated samples. Enrichments with either modified tryptic soy broth (mTSB) containing 8 mg L-1 novobiocin (mTSB-8) or modified Escherichia coli (mEC) broth followed by isolation in MacConkey agar were the most sensitive combinations for STEC isolation of artificially inoculated samples. Independently, both enrichments media followed by isolation in MacConkey were used to evaluate ground beef samples from 43 retail stores, yielding 65.1% and 58.1% stx-positive samples by RT-PCR, respectively. No difference were observed in the isolate proportions between these two methods (8/25, 32% and 8/28, 28.6%). Identical serotypes and stx genotypes were observed in STEC strains isolated from the same samples by either methods. In this study, no single enrichment protocol was sufficient to detect all STEC in artificially inoculated samples, and had considerable variation in detection ability with naturally contaminated samples. Moreover the usage of single or combination of multiple isolation agars were not capable of identifying all STEC serogroups in either artificially inoculated or naturally occurring STEC-contaminated ground beef. Therefore, it may be prudent to conclude that there is no single method or combination of isolation methods capable of identifying all STEC serogroups.