Analysis of possible genotoxicity of the herbicide flurochloridone andits commercial formulations: Endo III and Fpg alkaline comet assays in Chinese hamster ovary (CHO-K1) cells

LARRAMENDY, M; NIKOLOFF N; SOLONESKI, S

MUTATION RESEARCH. GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 797 p. 46 - 52

1383-5718

Cytotoxic and genotoxic effects of flurochloridone (FLC) and its formulations Twin Pack Gold®andRainbow®were evaluated in CHO-K1 cells. Using the alkaline single-cell gel electrophoresis (SCGE)assay, we observed that FLC (15 g/ml), Twin Pack Gold®or Rainbow®induced primary DNA dam-age, increasing the frequency of damaged nucleoids. Vitamin E pretreatment did not modify the effect.Decreased cell viability was observed only in Twin Pack Gold®-treated cultures and was significantlyameliorated by vitamin E. Post-treatment of herbicide-damaged CHO-K1 cells with the enzymes Endo IIIor Fpg did not increase FLC-, Twin Pack Gold®-, or Rainbow®-induced DNA damage. These results demon-strate that neither FLC nor FLC-based formulations induce DNA damage through hydroxyl radical or lipidalkoxyl radical production, and that the induced DNA lesions were not related to oxidative damage atthe purine/pyrimidine level. Our observations strongly suggest that the cytotoxic effects observed afterTwin Pack Gold®exposure are due to the excipients contained within the technical formulation ratherthan FLC itself.