Evaluación de los arándanos como radioprotectores potenciales

MARÍA DEL CARMEN MENÉNDEZ; ELISA EUGENIA CÓRDOBA; MARINA CONTARDI; ALBA MABEL GÜERCI

Perspectivas en Nutrición Humana

Universidad de Antioquía

Lugar: Medellín; Año: 2015 vol. 17 p. 11 - 19

0124-4108

Introduction: Numerous studies have analyzed the antioxidant capacity of blueberries. Considering the ionizing radiation cytotoxicity mediated by free radicals is imperative phytocompounds analysis with potential mitigating effect. Objective: To evaluate radio-protective properties of this fruit in relation to genetic damage induced by x-rays. Materials and methods: Experimental design had two stages. First an in vitro assay using 10 samples of peripheral blood of young and nonsmokers female. Each sample was analyzed by comet assay in the next set of treatments: negative control, treatment with blueberries (0,232 mG / mL), irradiation 4Gy and simultaneous blueberry/ irradiation treatment. Were counted 800 cells/individual, 200 per treatment, considering its repetition. Subsequently, an in vivo assay with peripheral blood of two women, of similar conditions and subject to the consumption of dried extract of blueberries for 15 consecutive days was performed. Sampling was performed before and after treatment and Comet was implemented by analyzing 800 cells / individual, corresponding to the negative control and irradiation with 4 Gy. Results: In both stages, treatment with blueberries showed a significant reduction (p