The importance of manganese in the cytoplasmic maturation of cattle oocytes: blastocyst production improvement regardless of cumulus cells presence during in vitro maturation

JUAN PATRICIO ANCHORDOQUY; JUAN MATEO ANCHORDOQUY; MATIAS ANGEL SIRINI; JUAN ALBERTO TESTA; PILAR PERAL-GARCÍA; CECILIA CRISTINA FURNUS

ZYGOTE

CAMBRIDGE UNIV PRESS

Lugar: Cambridge; Año: 2015

0967-1994

Adequate dietary intake of manganese (Mn) is required for normal reproductive performance incattle. This study was carried out to investigate the effect of Mn during in vitro maturation of bovinecumulus?oocyte complexes (COC) on apoptosis of cumulus cells, cumulus expansion, and superoxidedismutase (SOD) activity in the COC. The role of cumulus cells on Mn transport and subsequentembryo development was also evaluated. Early apoptosis decreased in cumulus cells matured withMn compared with medium alone. Cumulus expansion did not show differences in COC maturedwith or without Mn supplementation. SOD activity was higher in COC matured with 6 ng/ml Mn thanwith 0 ng/ml Mn. Cleavage rates were higher in COC and denuded oocytes co-cultured with cumuluscells, either with or without Mn added to in vitro maturation (IVM) medium. Regardless of the presenceof cumulus cells during IVM, the blastocyst rates were higher when 6 ng/ml Mn was supplementedinto IVM medium compared with growth in medium alone. Blastocyst quality was enhanced whenCOC were matured in medium with Mn supplementation. The results of the present study indicatedthat Mn supplementation to IVM medium enhanced the ?health? of COC, and improved subsequentembryo development and embryo quality.