Evaluación citomolecular del efecto vecindad en fibroblastos

A. GÜERCI; M. V. PONZINIBBIO; D. DODAT; M. MARTÍNEZ; SÁNCHEZ,; O. CASAS

Revista de Física Médica

Sociedad Española de Física Médica

Lugar: Madrid; Año: 2008 vol. 9 p. 19 - 24

Abstract During the last years one of the major paradigms of radiobiology has changed. Originally, it was belived that only those cells directly hit by radiation were the ones that could damage their genetic material. At present the idea of neighboring cells being at genotoxic risk is growing considerably. The aim of this research was to show the existance of this phenomenon, usually called bystander effect, specifically in human lung fibroblasts exposed to a dose which is frecuently used in fractioned radiotherapy. The experimental design consisted of treating non-irradiated cells with the culture medium coming from cells that had been previously irradiated (ICM). We recollected ICM formed during different times of incubation: 30, 60, 120 and 180 minutes. After that, non-irradiated cells were treated with these medium during one hour in all the cases. The damage in the genetic material was analyzed by the Comet assay. The results show considerable differences in the genomic damage among the cells treated with ICM and their corresponding control. A cyclic response in such levels has also been observed. This might be due to the disbalance between the proxidant and the antioxidant factors produced in response to radiation. These findings would contribute to optimize the implemented methodology both in radiotherapy  and radioprotection.