EVALUATION OF THE GENOMIC DAMAGE INDUCED BY

PONZINIBBIO MV,; CRUDELI C,; GÜERCI A.

BIOCELL

Lugar: Mendoza; Año: 2008 p. 134 - 134

0327-9545

The biological effects of ionizing radiation apparently respond to the impact on the irradiated material. However, there is evidence of damage in no-irradiated cells. This phenommenon- Bystander effect- involves the transmission of several molecules that act as signals from the irradiated culture medium (ICM) to the neighboring ones. Our aim was to corroborate this effect in MRC-5 line exposed to a radio therapeutic dose (200 cGy of X- ray). The methodology includes the treatment of non- irradiated cells with the ICM coming from cells that have been previously irradiated. This medium has been transferred to non- irradiated cells at four different times. The genotoxic damage detection was carried out through Comet assay: 200 images were evaluated by each end point and classified according to their level of damage. These data has been analyzed trough ÷2. We could observe induced damage from several factors present in the ICM. In the four evaluated instances, significant differences were found (p> 0,001) between the cells treated with the ICM and their corresponding control. The obtained results allow us to suppose that the cellular response to radiotherapeutic exposures suggest the joint action of the different mechanisms. A higher level of damage to that expected might be explained only by the direct action. These results might also support the fact that genetic and epigenetic factors are involves.