Allele Frequencies of 15 STR loci in the Quito city Population, Ecuador

GOMEZ M.A.; PEÑAHERRERA M. S.; AGUIRRE-TELLO V.; VELA-CAVINATO M.; GIOVAMBATTISTA G.

JOURNAL OF FORENSIC SCIENCES

Blackwell

Año: 2008 p. 510 - 511

0022-1198

Whole blood obtained by venipuncture was collected from unrelated individuals residing in Quito city, Ecuador, South America, using one 1.2mm micropunch of bloodstains collected on FTA Classisc Cards (Whatman FTAâ, USA), and the DNA were extracted following the manufacturer´s directions. PCR amplification of the fifteen STR loci (D3S1358, THO1, D21S11, D18S51, PENTA E, D5S818, D13S317, D7S820, D16S539, CSF1PO, PENTAD, VWA, D8S1179, TPOX, FGA) was performed using the PowerPlex®16 System kit (Promega Corp.,  Madison, WI, USA) (1) (PE-Biosystems, Foster City, CA, USA) following the manufacturer’s protocol. The amplified products were separated and detected using the ABI Prism® 310 genetic analyzer (Applied Biosystems, Foster City, CA, USA) using the ABI Prism GeneScan analysis software version 3.1 and Genotyper DNA fragment analysis software version 2.5.2 (2).             The data were analyzed using Power-Stats (3). Allele frequencies and other relevant information from analysis of the fifteen STR loci are given in Table 1. The complete data set is available to any interested researcher upon request, or can be download from the follow world wide web site URL: http://www.fcv.unlp.edu.ar/info-general/publicaciones/pdf/supplemtary.pdf.