IGEVET   21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
Effect of buthionine sulphoximine, cysteine and dithitreitol in cells exposed to low doses of radiation
Autor/es:
DE LUCA, J.C.; LOPEZ-LARRAZA D.M.
Revista:
Int J Low Radiation
Editorial:
Inderscience Publisher
Referencias:
Año: 2014 vol. 9 p. 327 - 334
ISSN:
1741-9190
Resumen:
The goal of this study is to determine whether cysteimine, buthionine sulphoximide and dithitreitol protect CHO K1 cells against chromosomal aberrations induced by low doses of X-radiation, as well as whether radiation protection is related to physical properties of those thiols. Experimental design included four different treatments: (a) control, (b) cells treated with 5 mM of buthionine sulphoximide as well as cysteine and dithitreitol, (c) cells treated with 100 mGy of X-rays and (d) cells treated with 5 mM of each thiol and 100 mGy X-rays. In combined treatment, all chemical compounds were added 30 min before irradiation and remained until cell sacrifice (18 h). None of the three compounds studied showed a radioprotector effect. These results are consistent with the electrical charges and chemical structure of the three thiols.