Comparison of the effectiveness between microsatellites and SNP panels for parentage and genetic identification in an inbred Angus herd

FERNANDEZ M E; GOSZCZYNSKI D E; LIRON J P; VILLEGAS CASTAGNASSO E E; CARINO M H; RIPOLI M V; ROGBERG MUÑOZ A; POSIK D M; PERAL GARCIA P; GIOVAMBATTISTA G

GENETICS AND MOLECULAR BIOLOGY

SOC BRASIL GENETICA

Año: 2013 vol. 36 p. 185 - 191

1415-4757

For Review O During  the  last  decade,  microsatellites  (STRs,  Short  Tandem  Repeats)  have  been successfully used in animal genetic identification, traceability and paternity, although.in the  last  few years single nucleotide polymorphisms (SNPs) have  increased popularity. An  efficient  SNP  identification  system  requires  a marker  set  with  enough  power  to identify  individuals and  their parents. Usually, genetic diagnostics  include  the analysis of  related  animals.  In  this  work,  the  degree  of  information  obtained  through  SNPs versus STRs within  a  consanguineous herd was  compared. Thirty6six  closely  related Angus were genotyped for 18 STRs and 116 SNPs. Cumulative SNPs exclusion power values  (Q)    for paternity, and sample matching  (MP)  resulted higher  than 0.9998 and 4.32E642,  respectively.  Between  2  and  3  SNPs  per  STR  were  needed  to  obtain  an equivalent Q  value. MP  showed  that  24  SNPs  are  equivalent  to  the  12 STRs  ISAG (International  Society  for  Animal  Genetics)  minimal  recommended  set  (MP~10611). These  results  provide  very  valuable  genetic  data  that  supports  the  consensus  SNP panel for bovine genetic identification developed by  the Parentage Recording Working Group of ICAR (International Committee for Animal Recording).