IGEVET 21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
Prolactin (PRL) induction of cyclooxygenase 2 (COX2) expression and prostaglandin (PG) production in hamster Leydig cells
Autor/es:
MATZKIN ME; AMBAO V; CARINO MH; ROSSI SP; GONZALEZ L; TURYN D; CAMPO S; CALANDRA RS; FRUNGIERI MB
Revista:
MOLECULAR AND CELLULAR ENDOCRINOLOGY.
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2012 vol. 348 p. 33 - 46
ISSN:
0303-7207
Resumen:
Serum prolactin (PRL) variations play a crucial role in the photoperiodic-induced testicular regression?recrudescence transition in hamsters. We have previously shown that cyclooxygenase 2 (COX2), a keyenzyme in the biosynthesis of prostaglandins (PGs), is expressed mostly in Leydig cells of reproductivelyactive hamsters with considerable circulating and pituitary levels of PRL. In this study, we describe astimulatory effect of PRL on COX2/PGs in hamster Leydig cells, which is mediated by IL-1b and preventedby P38-MAPK and JAK2 inhibitors. Furthermore, by preparative isoelectric focusing (IEF), we isolated PRLcharge analogues from pituitaries of active [isoelectric points (pI): 5.16, 4.61, and 4.34] and regressed (pI:5.44) hamsters. More acidic PRL charge analogues strongly induced COX2 expression, while less acidicones had no effect.Our studies suggest that PRL induces COX2/PGs in hamster Leydig cells through IL-1b and activation ofP38-MAPK and JAK2. PRL microheterogeneity detected in active/inactive hamsters may be responsible forthe photoperiodic variations of COX2 expression in Leydig cells.
