IGEVET 21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
Analysis of apoptosis and DNA damage in bovine cumulus cells after exposure in vitro to different zinc concentrations
Autor/es:
ANCHORDOQUY J M; PICCO S J; SEOANE A I; ANCHORDOQUY J P; PONZZINIBIO M V; MATTIOLI G A; PERAL GARCIA P; FURNUS C C
Revista:
CELL BIOLOGY INTERNATIONAL
Editorial:
ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD
Referencias:
Año: 2011 vol. 35 p. 593 - 597
ISSN:
1065-6995
Resumen:
The purpose of this study was to investigate the effect of Zn (zinc) concentration on CCs (cumulus cells) during in vitro maturation. For this purpose, DNA integrity of CCs by addition of different Zn concentrations [0 (control); 0.7 μg/ml (Zn1); 1.1 μg/ml (Zn2) and 1.5 μg/ml (Zn3)] to the culture medium was evaluated by comet assay. In addition, early apoptosis was analysed by annexin staining assay. CCs treated with Zn showed a significant decrease in the DNA damage in a dose-dependent manner. Comet assay analysed for TM (tail moment) was significantly higher in cells cultured without Zn (control, P
