IGEVET   21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
Effects of Glucose, Propionate and Splanchnic Hormones on Neuropeptide mRNA concentrations in the Ovine Hypothalamus
Autor/es:
A. E. RELLING,; LEE K; LOERCH S.C.; REYNOLDS C.K.
Revista:
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2012 vol. 96 p. 648 - 654
ISSN:
0931-2439
Resumen:
Hypothalamic tissue from 4 to 5 month old lambs was 23 obtained at slaughter and immediately incubated in culture media for 2 h at 36ºC. Treatments included a control Dulbecco´s Modified Eagle Medium (DMEM) containing 1 mM glucose or DMEM with the following additions: 10 mM glucose, 1 mM propionate, 1 nM insulin, 120 pM GLP-1, 100 pM PYY, 80 pM CCK, or 10 mM glucose plus 1 nM insulin. The abundance of mRNA for NPY, AgRP, and POMC was measured using quantitative reverse transcriptase PCR. Fisher´s protected LSD test was used to compare changes in relative mRNA concentrations for the hypothalamus incubated in the control media versus the rest of the treatments. The media containing glucose plus insulin increased POMC mRNA concentration (P < 0.05), but did not affect NPY or AgRP mRNA concentration. There were no effects observed for the other treatments (P > 0.20). Results of the present study support the hypothesis that effects of propionate on feed intake in ruminants is not  mediated through effects on the hypothalamus, and that insulin is required for an effect of glucose on hypothalamic POMC expresion.