IGEVET 21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
Genetic effects of low dose radiation: DNA damage and micronucleus in X-rays treated cells
Autor/es:
PONZZINIBIO M.V.; CRUDELI C.; PERAL GARCIA P.; SEOANE A.
Revista:
ACTA RADIOLOGICA
Editorial:
TAYLOR & FRANCIS LTD
Referencias:
Año: 2010 vol. 51 p. 1028 - 1033
ISSN:
0284-1851
Resumen:
Background: Exposure to environmental, diagnostic and occupational sources of radiation frequently involves low doses. Although these doses have no immediately noticeable impact on human health there is great interest in their long-term biological effects. Purpose: The purpose of the present study was to assess immediate and time-delayed DNA damage in two cell lines exposed to low doses of X-rays by using comet assay and micronucleus test, and to compare these two techniques in the analysis of low-dose induced genotoxicity. Material and Methods: CHO and MRC-5 cells were exposed to 50 miliSievert of X-rays and assayed immediately after irradiation and at 16 or 12 passages post-irradiation respectively. Comet assay and micronucleus test were employed. Results: The comet assay values observed in 50 mSv-treated cells were significantly higher than in the control group at both sample times and cell lines (p
