INVESTIGADORES
ALVAREZ Luis Ignacio
congresos y reuniones científicas
Título:
Differential exposure of Haemonchus contortus to albendazole according to the site of worm location in the abomasum of infected sheep
Autor/es:
ALVAREZ, L.; BARRERE, V.; SUAREZ, G.; CEBALLOS, L.; MORENO, L.; PRICHARD, R., LANUSSE, C.
Lugar:
Buenos Aires
Reunión:
Conferencia; 23 International Conference of the World Association for the Advancement of Veterinary Parasitology; 2011
Institución organizadora:
Asociacón Argentina de Parasitología Veterinaria
Resumen:
The gastrointestinal absorption of most drugs follows a first-order kinetics, whereby a constant fraction of the total drug is absorbed in each equal interval of time. This is applicable to the most of the therapeutically used drugs, but remains unclear for the poorly water soluble benzimidazole (BZD) anthelmintics in ruminants. The main goals of the current trial were: a) to characterize the albendazole (ABZ) metabolites plasma disposition kinetics after ABZ administration at different dose rates to lambs, b) to compare the clinical efficacy of different ABZ dose levels in lambs infected with a BZD-resistant strain of Haemonchus contortus, and c) to analyze the frequency of mutations at three different single nucleotide polymorphisms (SNP) on the beta-tubulin isotype 1 encoding gene in worms surviving the treatments at different dosages. Twenty four (24) Corriedale lambs artificially infected with a resistant H. contortus strain were allocated into 4 groups (n=6) and intraruminally treated with ABZ at either 5 (ABZ5), 15 (ABZ15) or 45 (ABZ45) mg/kg. Plasma samples were collected up to 120 h post-treatment and analyzed by HPLC. An untreated control Group was included to assess the comparative anthelmintic efficacy of the different treatments against H. contortus. Additionally, adult specimens of H. contortus were recovered directly from the abomasum for genetic analysis. Parametric and non-parametric tests were used to compare the statistical significance of the results (P