An economical and high throughput alternative for endoglucanase activity determination.

ORTIZ GE; BLASCO M.; ALBERTÓ E.

Biotechnology an Indian Journal

Trade Science Inc

Año: 2016 vol. 12 p. 70 - 74

0974-7435

The search for new strains with good productivity of cellulolytic enzymesis always necessary to improve the processes are usually employed.Screening protocols used for these purposes require a low cost and highthroughput assay for determination of endoglucanase activity. However,the test proposed by the IUPAC and other microadaptations not meet thisrequirement. The aim of our work is to obtain an economical and high-performance proposed by IUPAC for determination of endoglucanaseactivity assay adaptation. For this we reduced the assay volume andinvestigate the effect of this reduction on endoglucanase activitydetermination. As a result a miniaturized assay statistically correlated withthe assay proposed by IUPAC reduced 10-fold lower scale was obtained.As a result a new economic and high performance adaptation forendoglucanase activity assay based on the IUPAC protocol was obtained