INVESTIGADORES
ALBERTO Edgardo Omar
artículos
Título:
Ornithine and arginine decarboxylase activities and effect os some polyamine biosíntesis inhibitors on Gigaspora rosea germinating spores
Autor/es:
SANNAZZARO, A. I; C. L. ALVAREZ; A. B. MENÉNDEZ; F. L. PIECKENSTAIN; E. ALBERTÓ; O. A. RUIZ
Revista:
FEMS MICROBIOLOGY LETTERS
Editorial:
Blackwell publishing
Referencias:
Año: 2004 vol. 230 p. 115 - 121
ISSN:
0378-1097
Resumen:
The pathways for putrescine biosynthesis and the effects of polyamine biosynthesis inhibitors on the germination and hyphal development of Gigaspora rosea spores were investigated. Incubation of spores with different radioactive substrates demonstrated that both arginine and ornithine decarboxylase pathways participate in putrescine biosynthesis in G. rosea.Spermidine and spermine were the most abundant polyamines in this fungus. The putrescine biosynthesis inhibitors á-difluoromethylarginine and á- difluoromethylornithine, as well as the spermidine synthase inhibitor cyclohexylamine slightly decreased polyamine levels. However, only the latter decreased spore germination. The consequences of the use of putrescine biosynthesis inhibitors for the control of plant pathogenic fungi on the viability of G. rosea spores in soil are discussed.biosynthesis inhibitors on the germination and hyphal development of Gigaspora rosea spores were investigated. Incubation of spores with different radioactive substrates demonstrated that both arginine and ornithine decarboxylase pathways participate in putrescine biosynthesis in G. rosea.Spermidine and spermine were the most abundant polyamines in this fungus. The putrescine biosynthesis inhibitors á-difluoromethylarginine and á- difluoromethylornithine, as well as the spermidine synthase inhibitor cyclohexylamine slightly decreased polyamine levels. However, only the latter decreased spore germination. The consequences of the use of putrescine biosynthesis inhibitors for the control of plant pathogenic fungi on the viability of G. rosea spores in soil are discussed.