Efficiency of treatments for controlling Trichoderma sp during spawning in mushroom cultivation

COLAVOLPE, M. B.; JARAMILLO, S.; ALBERTÓ E.

BRAZILIAN JOURNAL OF MICROBIOLOGY

SOC BRASILEIRA MICROBIOLOGIA

Año: 2014 vol. 45 p. 1263 - 1270

1517-8382

Trichoderma spp is the cause of the green mold disease in mushroom cultivation production. Manydisinfection treatments are commonly applied to lignocellulose substrates to prevent contamination.Mushroom growers are usually worried about the contaminations that may occur after these treat-ments during handling or spawning. The aim of this paper is to estimate the growth of the green moldTrichoderma spon lignocellulose substrates after different disinfection treatments to know which ofthem is more effective to avoid contamination during spawning phase. Three different treatmentswere assayed: sterilization (121 °C), immersion in hot water (60 and 80 °C), and immersion in alka-linized water. Wheat straw, wheat seeds and Eucalyptus or Populussawdust were used separately as substrates. After the disinfection treatments, bagged substrates were sprayed with 3 mL of suspen-sion of conidia of  richoderma sp (10 5conidia/mL) and then separately spawned withPleurotus ostreatus or Gymnopilus pampeanus. The growth of Trichoderma spwas evaluated based on a quali-tative scale. Trichoderma sp could not grow on non-sterilized substrates. Immersions in hot water treatments and immersion in alkalinized water were also unfavorable treatments for its growth. Co-cultivation with mushrooms favored Trichoderma sp growth. Mushroom cultivation disinfection treatments of lignocellulose substrates influence on the growth of Trichodermasp when contamina-tions occur during spawning phase. The immersion in hot water at 60 °C for 30 min or in alkalinized water for 36 h, are treatments which better reduced the contaminations withTrichodermasp during spawning phase for the cultivation of lignicolous species.