INVESTIGADORES
ALBERTO Edgardo Omar
artículos
Título:
Microplate assay for endo-polygalacturonase activity determination based on ruthenium red method.
Autor/es:
ORTÍZ, G.; GUITART, M. E. ; ALBERTÓ E.; FERNÁNDEZ LAHORE, H. M. ; BLASCO M.
Revista:
ANALYTICAL BIOCHEMISTRY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 454 p. 33 - 35
ISSN:
0003-2697
Resumen:
Endo-polygalacturonase (endo-PGase) activity determinations generally rely on viscosity changes or reducing sugar ends produced by this activity over polygalacturonic acid. Torres and coworkers [EnzymeMicrob. Technol. 48 (2011) 123?128] showed that ruthenium red (RR) is useful for endo-PGase determi-nation. In this article, we present a high-throughput liquid-based endo-PGase assay based on the RRmethod and compare it with the viscosity determination method. The reduced assay uses a small volume of enzyme solution, 40lg of polygalacturonic acid, and 45 lg of RR for each sample determination. Furthermore, we obtained an interconversion factor for RR and viscosity activities.
