INVESTIGADORES
POSE Graciela Noemi
artículos
Título:
Taxonomic Characterization and Secondary Metabolite Profiling of Aspergillus Section Aspergillus Contaminating Feeds and Feedstuffs
Autor/es:
GRECO M.; KEMPPAINEN M; POSE G.; PARDO A.
Revista:
Toxins
Editorial:
MDPI AG
Referencias:
Año: 2015 vol. 7 p. 3512 - 3537
ISSN:
2072-6651
Resumen:
Xerophilic fungal species of the genus Aspergillusare economically highly relevant due to their ability to grow on low wateractivity substrates causing spoilage of stored goods and animal feeds. Thesefungi can synthesize a variety of secondary metabolites many of which showanimal toxicity, generating health risk for food production animals and tohumans as final consumers respectively. Animal feeds used for rabbit,chinchilla and rainbow trout production in Argentina were analysed for thepresence of xerophilic Aspergillussection Aspergillus species. High isolationfrequencies (>60 %) were detected in all the studied rabbit and chinchillafeeds, while the rainbow trout feeds showed lower fungal charge (25%). Thesesection Aspergillus contaminations comprisedpredominantly five taxa. Twenty isolates were subjected to taxonomiccharacterization using both ascospore SEM micromorphology and two independentDNA loci sequencing. The secondary metabolite profiles of the isolates weredetermined qualitatively by HPLC-MS. All the isolates produced neoechinulin A,17 isolates were positive for cladosporin and echinulin, and 18 were positivefor neoechinulin B. Physcion and preechinulin were detected in a minorproportion of the isolates. This is the first report describing the detailedspecies composition and the secondary metabolite profiles of Aspergillus section Aspergilluscontaminating animal feeds.