INVESTIGADORES
PODEROSO Juan Jose
congresos y reuniones científicas
Título:
Oxidative Stress in skeletal muscle during murine sepsis
Autor/es:
PODEROSO JJ; LLESUY S; EVELSON P; PERALTA JG; CARRERAS MC
Lugar:
Buenos Aires
Reunión:
Simposio; Simposio Internacional Active Oxygen Species and Human Health.; 1991
Resumen:
Skeletal muscle is a target organ during sepsis; there are no evidences about a possible free radical overproduction with tissue damage by lipoperoxidation in this situation. We studied Sprague Dawley female rats in two groups: a septic group with cecal ligation and double cecal perforation and a control one, sham operated. Hind limb adductor muscles spontaneous chemiluminescence was measured at 2, 4, 6, 12, 24 and 30 h after the surgical procedure; also muscle samples were obtained and enzymatic activity of superoxide dismutase, catalase and glutathione peroxidase were determined. The results showed that light emission by rat skeletal muscle duplicated from 4 to 12 h of sepsis which is attributable to the production of oxygen free radicals. The detoxifying antioxidant enzymes were found to be diminished (MnSOD 46% at 6h, catalase 83% at 12h, glutathione peroxidase 55% at 12h), which in turn should facilitate muscle septic damage.