INVESTIGADORES
PODEROSO Juan Jose
congresos y reuniones científicas
Título:
Chronology of oxidative stress in duodenal reflux pancreatitis in rats
Autor/es:
PERALTA JG; REIDES C; PARGAMENT GA; LLESUY S; CARRERAS MC; PODEROSO JJ
Lugar:
Pasadena, EEUU
Reunión:
Congreso; Annual Meeting of the Oxygen Society. Oxygen 1995.; 1995
Resumen:
Oxidative stress plays a role in different models of acute pancratitis, but it has not been studied in closed duodenal loop pancreatitis. In this setting, our objective was to determine the chronology of spontaneous chemiluminescence (CHL) from pancreas and liver and, to relate it to pancreatic damage and circulating antioxidant defences. Pancreatitis was produced by duodenal ligation and CHL was detected by means of a Thorn-EMI phototube at 0, 0.5, 1.5, 3.6, 12 and 24h; Trolox equivalent peroxyl trapping capability of plasma was measured (Wayner’s method) and blood amylase, bilirrubin, and wet/dry weight ratio of pancreatic tissue were determined in rats with pancreatitis (P) and in sham operated animals (C). Bovine superoxide dismutase (SOD) (15 mg/kg i.v.) was administered as a diagnostic and therapeutic tool. Significant light emission was detected in P vs C from the liver at 3 hr: 29± 6 vs 15±6 cps/cm2 (n=6, p<0.05), and from pancreas at 6hr: 35±2 vs 25±2 cps/cm2 (n=6, p<0.05), both peaked at 12 hr and returned to control levels at 24 hr. SOD attenuated 59% light emission (p<0.05) at 6 hr but not amylase or pancreatic edema. Plasma antioxidant capacity increased with time, was maximal at 12 hr and appeared related to bilirrubin levels (n=20) (r: 0.73, p<0.05) and then decreased at 24 hr, probably denoting consumption of other antioxidants. We conclude that, in this obstructive model, production of oxygen excited species emerges earlier in the liver than in the pancreas, while plasma antioxidant capacity is unexpectedly increased even up to the late stages and, SOD results insufficient as isolated therapy.