INVESTIGADORES
PONZIO Marina Flavia
congresos y reuniones científicas
Título:
Monitoring ovarian activity in jaguar (Panthera onca): determination of estrogen and progesterone fecal metabolite levels.
Autor/es:
CANTARELLI V; MASTROMONACO G; ROSAS AC; CARRO NG; NOYA MR; PENA MARTINEZ J,; PONZIO MF
Lugar:
Orlando, FL
Reunión:
Conferencia; 6th Conference of the International Society of Wildlife Endocrinology.; 2017
Institución organizadora:
International Society of Wildlife Endocrinology.
Resumen:
One of themajor concerns for the Jaguar conservation is the increase of habitat loss,fragmentation and hunting. The species has been virtually eliminated from muchof its historical range. With the severe decline of the wild populations, itbecame increasingly important understanding the species basic reproductive physiologyfor a successful conservation program.We determinedfecal estrogen and progesterone metabolite levels of a female jaguar maintainedin captive conditions at the ?Jaguar Experimental Breeding Center? from The ConservationLand Trust, in the wetlands of Ibera Natural Reserve, Corrientes, Argentina. Amale was allowed to mate when the female showed external signs of estrus. Fecalsamples were collected daily from April-December of 2016 (n=208). Fecal extracts(0.5g wet feces in 80% methanol, 24 h extraction at 4°C) were analyzedby EIA using the Estradiol 17â-R0008 and Progesterone-R4859 antibodies (Coralie Munro, UC Davis, USA).Mean durationof the ovarian cycle (defined as the number of days between two consecutive estrogenpeaks) of was 32.7±2.0 days (range: 28?37 days; n=4 estrous cycles). Mean estrous length was6.2±0.4 days andluteal phase was 19.5±4.1 days, defined by a lapse of sustained high levels of progesterone(range: 11?27 days; n=4). Baseline concentrations of estradiol and progesterone were 9.5±0.5 and 137.1± 9.8 ng/g wet feces respectively. Peak fecal estradiol and progesteroneconcentrations reached values of 218.4±59.1 and 1330.1±113.0 ng/g wet feces, respectively.The hormonaland behavioural data suggested that this female ispolyestrous and that ovulation was not linked to the events of copulationalone, suggesting that spontaneous ovulation occurs regularly. This protocolwas effective for monitoring the ovarian cycle in the jaguar, and the ovariancycle duration and estrous length were coincident with the scarce data on thisspecies.