INVESTIGADORES
PONZIO Marina Flavia
artículos
Título:
Semen quality and neutral alpha-glucosidase activity after sequential electroejaculation in the chinchilla (Ch. lanigera).
Autor/es:
PONZIO M.F; ROUSSY OTERO GN; RUIZ R.D,; FIOL DE CUNEO M
Revista:
ANIMAL REPRODUCTION SCIENCE
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2011 vol. 126 p. 229 - 233
ISSN:
0378-4320
Resumen:
The objectives of this study of seminal quality of chinchilla (Chinchilla lanigera) males
were to evaluate (1) the use of neutral alpha-glucosidase (NAG) as a functional epididymal
marker in this species, and (2) the effects of repetitive electroejaculation on sperm function
and seminal NAG concentration. Semen was obtained by electroejaculation from sexually
mature domestic Ch. lanigera males (n = 6) once a week for six consecutive weeks, and evaluated
to assess seminal volume and NAG activity, and sperm concentration and functional
activity (motility, viability, membrane and acrosome integrity). Body weight was determined
in all animals before each electroejaculation procedure. NAG activity was detected
in all semen samples obtained, reaching a concentration of 55.4±6.8 U/mL. No significant
variations were observed throughout the experimental period in any of the semen quality
parameters evaluated. A reduction (P = 0.001) in body weight was detected from the third
week of experimentation and by the end of the experiments the animals lost a 7.2±0.9% of
body weight. Overall, results in the present study confirm that NAG activity is detectable
in chinchilla seminal plasma and that repetitive electroejaculation is not deleterious, on a
short-term basis, to the semen characteristics.Chinchilla lanigera) males
were to evaluate (1) the use of neutral alpha-glucosidase (NAG) as a functional epididymal
marker in this species, and (2) the effects of repetitive electroejaculation on sperm function
and seminal NAG concentration. Semen was obtained by electroejaculation from sexually
mature domestic Ch. lanigera males (n = 6) once a week for six consecutive weeks, and evaluated
to assess seminal volume and NAG activity, and sperm concentration and functional
activity (motility, viability, membrane and acrosome integrity). Body weight was determined
in all animals before each electroejaculation procedure. NAG activity was detected
in all semen samples obtained, reaching a concentration of 55.4±6.8 U/mL. No significant
variations were observed throughout the experimental period in any of the semen quality
parameters evaluated. A reduction (P = 0.001) in body weight was detected from the third
week of experimentation and by the end of the experiments the animals lost a 7.2±0.9% of
body weight. Overall, results in the present study confirm that NAG activity is detectable
in chinchilla seminal plasma and that repetitive electroejaculation is not deleterious, on a
short-term basis, to the semen characteristics.Ch. lanigera males (n = 6) once a week for six consecutive weeks, and evaluated
to assess seminal volume and NAG activity, and sperm concentration and functional
activity (motility, viability, membrane and acrosome integrity). Body weight was determined
in all animals before each electroejaculation procedure. NAG activity was detected
in all semen samples obtained, reaching a concentration of 55.4±6.8 U/mL. No significant
variations were observed throughout the experimental period in any of the semen quality
parameters evaluated. A reduction (P = 0.001) in body weight was detected from the third
week of experimentation and by the end of the experiments the animals lost a 7.2±0.9% of
body weight. Overall, results in the present study confirm that NAG activity is detectable
in chinchilla seminal plasma and that repetitive electroejaculation is not deleterious, on a
short-term basis, to the semen characteristics.±6.8 U/mL. No significant
variations were observed throughout the experimental period in any of the semen quality
parameters evaluated. A reduction (P = 0.001) in body weight was detected from the third
week of experimentation and by the end of the experiments the animals lost a 7.2±0.9% of
body weight. Overall, results in the present study confirm that NAG activity is detectable
in chinchilla seminal plasma and that repetitive electroejaculation is not deleterious, on a
short-term basis, to the semen characteristics.P = 0.001) in body weight was detected from the third
week of experimentation and by the end of the experiments the animals lost a 7.2±0.9% of
body weight. Overall, results in the present study confirm that NAG activity is detectable
in chinchilla seminal plasma and that repetitive electroejaculation is not deleterious, on a
short-term basis, to the semen characteristics.±0.9% of
body weight. Overall, results in the present study confirm that NAG activity is detectable
in chinchilla seminal plasma and that repetitive electroejaculation is not deleterious, on a
short-term basis, to the semen characteristics.