INTEQUI   20941
INSTITUTO DE INVESTIGACIONES EN TECNOLOGIA QUIMICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
"Comparative assays of cytotoxicity induced by extracts of Verbascum thapsus"
Autor/es:
ESCOBAR F.; GALLOTTI V.; SALAS M.; SABINI C.; GIORDANO O.; CONTIGIANI M.; SABINI L.
Lugar:
Huerta Grande. Córdoba. Argentina
Reunión:
Otro; Reunión Conjunta de Sociedades de Biología de la República Argentina, XVI Jornadas Científicas de la Sociedad de Biología de Córdoba, IX Jornadas de la Sociedad Argentina de Biología y XXV Reunión Anual de la Sociedad de Biología de Cuyo; 2007
Resumen:
Introduction:
The biotechnological application of V. thapsus in the phytomedicinal field is
promissory to control the microbial infections, which demands to study its
selectivity of action. Objective: To analyze the cytotoxic effect
of 5 vegetal extracts (VE) obtained from Verbascum thapsus (Ambay). The
extracts: n-hexanic (HE), chloroformic (CE), methanolic (ME),
cold and warms aqueous extract (CAE and WAE), were obtained from leaves of the
plant, by continuous and sequential agitation. The toxicity assays were carried
out in plates of 96 wells; 0.2 mL/well of
different concentrations of each VE [10-4000 ìg/ml] were
seeded on monolayer Vero cells. Controls wells without VE were included. The
system was incubated to 37°C by 48 hs. The
Maximum non Cytotoxic
Concentration (MCNC) was determinate by daily examination at the optical
microscopic. The CC50 was calculated by neutral red uptake method (NRU).
Statistical analysis of lineal regression was made, for a coefficient of
determination 0.90 (software Origin 7,5). Although by both techniques the toxic
concentrations surpassed 1mg/ml, NRU showed to be more sensitive to evaluate
toxicity on eukaryotic cells. A toxicity order was established: WAE <ME
<CAE <HE <CE. Data stimulate further bioactivities studies with these
extracts with the security that they not exerting cellular damage.