INTEQUI   20941
INSTITUTO DE INVESTIGACIONES EN TECNOLOGIA QUIMICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Differentiation of medicinal samples of Fumaria by FTIR and HPLC methods
Autor/es:
GARRO M.F.; FUNES M.D.; CAMI G. ; SAAD J.R.; PETENATTI M.E.; PETENATTI E.M. ; DEL VITTO L.A.
Lugar:
Mendoza
Reunión:
Congreso; XXXI Reunión Anual de la Sociedad de Biología de Cuyo; 2014
Resumen:
In order to expand the identification chances of some medicinal plant species by simple, rapid and inexpensive methods, aqueous extract of dried and milled plant samples of Fumaria officinalis L. and F. capreolata L. (Fumariaceae) underwent to FTIR and HPLC analysis. The first species is known as "fumaria" or "palomita" and is used as anticholinergic, antispasmodic, antiarrhythmic and bile flow regulator; the second is called "flor de la palomita" or "palomita", being used as a tonic, depurative, choleretic and skin diseases. IR spectra were obtained with a FTIR Protégé 460 spectrophotometer with CsI beamsplitter, acting upon pelletized drug (KBr 3% w/w), at a spectral resolution of 4 cm-1 in the range 4,000 and 460 cm-1. HPLC profiles were obtained with a Waters chromatograph provided with a diode array detector (DAD) and a C18 column, using the mobile phase acetonitrile:water (70:30). Obtained data were subsequently subjected to chemometrics methods, mainly the principal component analysis (PCA). FTIR spectra and HPLC chromatograms showed significant differences among species, such as the areas under the most representative absorption bands. Therefore, the profiles can be useful as "fingerprints" for the differentiation of these species, in order to contribute to an effective quality control of raw drugs as well as herbal medicines involved.