INTEQUI   20941
INSTITUTO DE INVESTIGACIONES EN TECNOLOGIA QUIMICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
AISLAMIENTO DE CEPAS NATIVAS DE HONGOS ENTOMATOGENOS PARA EL CONTROL DE Varroa destructor
Autor/es:
FERNANDEZ, CECILIA ; EGUARAS, MARTIN; PONZI, MARTA
Lugar:
Mar del Plata
Reunión:
Congreso; X CONGRESO ARGENTINO DE MICROBIOLOGIA GENERAL SAMIGE; 2014
Resumen:
Varroa destructor Anderson and Trueman (Acari: Varroidae) is an obligate ectoparasite of the western honey bee, Apis mellifera L. (Hymenoptera: Apidae). This mites feed on honeybee haemolymph, and transmit diseases that reduce bee longevity, lower reproductive capacity, and induce deformities. At present, beekeepers attempt to control varroa with chemical pesticides, but resistance to these chemicals is developing. The biological control of varroa warrants attention as a partial or total alternative to chemicals, and it could form part of an integrated pest management system. Fungal species that infect mites and insects are known as entomopathogens. Because the temperature within the honey bee hive is determined by ambient conditions, it would be logical to screen local isolates of fungi that fall within the ambient environmental conditions. This study report natural occurrence of entomopathogenic fungi pathogenic towards local Varroa destructor. Varroa mites were collected from experimental apiary of the National University of San Luis located in Villa Mercedes, San Luis, Argentina. Apiary hives were provided with technical floors to collect mites. After disinfection, specimens were incubated in a humids chambers at 100% of relative humidity for 48 hours and fungal growth was observed. In the colonies where fungal growth were plated on Potato dextrose agar (PDA) medium and incubated at 30 °C for 3-4 days. At the end of the growth period, a single colony was transferred onto another PDA agar medium and incubated at 30 °C, and the resultant mycelium was used to identification. Microscopic features observed are consistent with Entomomophthorales (Zygomycetes).