INTEQUI   20941
INSTITUTO DE INVESTIGACIONES EN TECNOLOGIA QUIMICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Isolation of an antiprotozoal flavonoid from stevia satureiifolia var. satureiifolia
Autor/es:
MARÍA FLORENCIA BEER; LAURA C. LAURELLA; ORLANDO ELSO; FERNANDA M. FRANK; VIRGINIA S. MARTINO; MARIA R. ALONSO; SILVIA L CAZORLA; EMILIO MALCHIODI; VALERIA P. SÜLSEN
Lugar:
Mar del plata
Reunión:
Congreso; X congreso de protozoologia y enfermedades parasitarias; 2014
Institución organizadora:
Sociedad Argentina de protozoología
Resumen:
Parasitic
diseases such as Chagas' disease and Leishmaniasis, affect millions of people
mainly in developing countries. These parasitosis are part of a group of diseases considered neglected,
abandoned and poverty-related. Drugs available to treat these protozoal
diseases have limitations due to adverse effects, emerging resistance and lack
of effectivity.
Taking in account this problem and the previous
isolation of a trypanocidal flavonoid from Stevia
satureiifolia var. satureiifolia organic extract, the aim of this work was
to isolate other bioactive compounds that may be present in this species.
The organic extract of S. satureiifolia was subjected to a bioassay-guided fractionation. From one of the most
active fraction (F2), a compound (compound 1) was isolated. Compound 1 was
evaluated against T. cruzi and L. braziliensis. This compound was
active against epimastigotes and trypomastigotes of T. cruzi with IC50 values of 0.65 µg/ml and 58.79 µg/ml, respectively. When compound 1 was tested on
promastigotes of L. braziliensis, it showed an IC50 value
of 6.77 µg/ml. This
compound showed no toxicity to mammalian cells. Chromatographic behavior and UV analysis
indicated that compound 1 could be a 5-hydroxy-tetra
substituted flavone. Further analyses are being conducted in order to complete
the identification of compound 1 and to determine its effects on the
intracellular forms of the parasites.