INTEQUI   20941
INSTITUTO DE INVESTIGACIONES EN TECNOLOGIA QUIMICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Isolation of an antiprotozoal flavonoid from stevia satureiifolia var. satureiifolia
Autor/es:
MARÍA FLORENCIA BEER; LAURA C. LAURELLA; ORLANDO ELSO; FERNANDA M. FRANK; VIRGINIA S. MARTINO; MARIA R. ALONSO; SILVIA L CAZORLA; EMILIO MALCHIODI; VALERIA P. SÜLSEN
Lugar:
Mar del plata
Reunión:
Congreso; X congreso de protozoologia y enfermedades parasitarias; 2014
Institución organizadora:
Sociedad Argentina de protozoología
Resumen:
Parasitic diseases such as Chagas' disease and Leishmaniasis, affect millions of people mainly in developing countries. These parasitosis are part of a group of diseases considered neglected, abandoned and poverty-related. Drugs available to treat these protozoal diseases have limitations due to adverse effects, emerging resistance and lack of effectivity. Taking in account this problem and the previous isolation of a trypanocidal flavonoid from Stevia satureiifolia var. satureiifolia organic extract, the aim of this work was to isolate other bioactive compounds that may be present in this species. The organic extract of S. satureiifolia was subjected to a bioassay-guided fractionation. From one of the most active fraction (F2), a compound (compound 1) was isolated. Compound 1 was evaluated against T. cruzi and L. braziliensis. This compound was active against epimastigotes and trypomastigotes of T. cruzi with IC50 values of 0.65 µg/ml and 58.79 µg/ml, respectively. When compound 1 was tested on promastigotes of L. braziliensis, it showed an IC50 value of 6.77 µg/ml. This compound showed no toxicity to mammalian cells. Chromatographic behavior and UV analysis indicated that compound 1 could be a 5-hydroxy-tetra substituted flavone. Further analyses are being conducted in order to complete the identification of compound 1 and to determine its effects on the intracellular forms of the parasites.