3. “Implementation of carbon-based nanomaterials in capillary electrochromatography as an immunosupport”

GERMÁN A. MESSINA; LORENA L. SOMBRA; WALTER P. STEGE; JULIO RABA; ROBERTO A. OLSINA; PATRICIA W. STEGE

Baltimore

Congreso; ITP 2012—19th International Symposium, Exhibit & Workshops on Electro- and Liquid Phase-separation Techniques; 2012

During the last twenty years the synthesis of a great variety of nanoparticles allows the application of nanotechnology to capillary electrophoresis. The usefulness of these nanostructurated particles are applicable to this method as a stationary or pseudostationary phase depending, basically, on the solubility of each nanoparticle. According to our experience carbon-based nanoparticles have many advantages to be used as not only a support but also as a platform to design new modifications. On the other hand, important advantages shown by capillary electrophoresis to study the specific interaction between biological molecules have placed this methods among the most utilized to measure this type of interactions. The aim of this study was focused in the application of carbon-based nanomaterial as an immunosupport in an affinity capillary electrochromatography applied to the determination of hepatitis B core antibody (anti-HBc) in human serum. The HBc antigen was attached to the nanoparticles and then several incubations were carried out as follow: human serum for 10 min, conjugate (antibody labeled with a peroxidase enzyme) for 10 min, and the last step was the substrate of the enzyme for 5 s. Between all the steps a 5 min washing was included. The modification of the capillary inner wall was made using multiwall and single wall carbon nanotubes and nanodiamonds. Every modification was evaluated following the variation on the EOF movement using DMSO 0.1% as a marker. Moreover, to bear out the presence of nanoparticles on de inner surface of the capillaries a SEM microscopy images were taken. Comparing the behavior of the different nanoparticles used in this study the most reproducible, stable on time and robust results were obtained with nanodiamonds. According to the obtained results we postulate that the variation in the behavior of the nanoparticles is based on the different hybridizations of the carbon in the particles which affect in different ways the interactions with the biologic molecules.