INTEQUI 20941
INSTITUTO DE INVESTIGACIONES EN TECNOLOGIA QUIMICA
Unidad Ejecutora - UE
artículos
Título:
An experimental Model of Affinity Filtration for the Isolation of Egg White Lysozyme using Cibacron Blue Immobilized to Yeast Cells.
Autor/es:
CARLOS AGUILAR; ULISES GONZALEZ; MARÍA DEL PILAR FERRARIS; JORGE RODRÍGUEZ
Revista:
JOURNAL OF CHROMATOGRAPHY B
Editorial:
ELSEVIER
Referencias:
Lugar: Amsterdam; Año: 2016 vol. 1020 p. 129 - 133
ISSN:
0378-4347
Resumen:
An experimental model of affinity filtration process was designed using a macroligand composed by Cibacron Blue F3GA immobilized to yeast cells. Its performance was evaluated, at bench scale, throug hthe recovery of egg white Lysozyme. The selective and reversible binding between the Cibacron ligand molecule and the enzyme is described. The separation of Lysozyme from the protein mixture included the application of stages such as affinity adsorption, concentration, diafiltration and elution. A tangential microfiltration system with an inorganic membrane was designed. The main finding was the development of the diafiltration operation, key stage in the enzyme isolation. The macroligand particle kept its integrity along the whole process and the degree of purity of the isolated Lysozyme was significant.
