INTEQUI 20941
INSTITUTO DE INVESTIGACIONES EN TECNOLOGIA QUIMICA
Unidad Ejecutora - UE
artículos
Título:
A study of Raphanus sativus and its endophytes as carbonyl group bioreducing agents
Autor/es:
PAULA RODRIGUEZ; CYNTHIA MAGALLANES-NOGUERA; PILAR MENÉNDEZ; ALEJANDRO ORDEN; DAVID GONZALEZ; MARCELA KURINA-SANZ; SONIA RODRIGUEZ
Revista:
BIOCATALYSIS AND BIOTRANSFORMATION
Editorial:
TAYLOR & FRANCIS LTD
Referencias:
Lugar: Londres; Año: 2015 vol. 33 p. 121 - 129
ISSN:
1024-2422
Resumen:
The reported anti-Prelog selectivity in Raphanus sativus hairy roots prompted us to search for similaractivity in other R. sativus systems, such as in vitro germinated sprouts, edible roots and its associatedendophytic microorganisms. Two test substrates were used in these studies: acetophenone and rac-ethyl 2-ethyl-3-oxobutanoate. Endophytes were isolated under substrate selective pressure and individual strainswere evaluated on their ability to reduce both substrates. Among the isolates, two bacteria and a funguspossessing potentiality to be used as biocatalysts were found: Bacillus megaterium that reducesacetophenone with excellent anti-Prelog selectivity, and Pseudomonas sp. and Penicillium chrysogenumwith enantioselectivity and stereoselectivity for the reduction of rac-ethyl 2-ethyl-3-oxobutanoate,respectively.
