INTEQUI   20941
INSTITUTO DE INVESTIGACIONES EN TECNOLOGIA QUIMICA
Unidad Ejecutora - UE
artículos
Título:
Mass spectrometry studies of Lycopodium alkaloids lycodine-type: Sauroxine and N-Demethyl-sauroxine
Autor/es:
CIFUENTE D.A.
Revista:
RAPID COMMUNICATIONS IN MASS SPECTROMETRY : RCM.
Editorial:
JOHN WILEY & SONS LTD
Referencias:
Lugar: LOndres; Año: 2014 vol. 28 p. 2690 - 2694
ISSN:
0951-4198
Resumen:
ABSTRACT RATIONALE: Sauroxine and N-Demethyl-sauroxine are Lycopodium alkaloids lycodine-type. During the last years Lycopodium alkaloids have gained a significant interest due to their unique skeletal characteristics as well as due to their acetylcholinesterase activity. Is known that drugs that inhibit acetylcholinesterase, can be used to treat early stages of Alzheimer disease. METHODS: Sauroxine and N-Demethyl-sauroxine were isolated from aerial parts of Huperzia saururus (Lam.) Trevis. Electron ionization mass spectrometry (EI-MS) (Low Resolution) and collision-induced dissociation tandem mass spectrometry in time (CID-MS/MS) fragmentation was conducted by an ion trap, GCQ Plus mass spectrometry with MS/MS. EIhigh- resolution mass spectrometry (EI-HRMS) was performed in a magnetic sector mass spectrometer Micromass VG. RESULTS: Using GC/EI-CID-MS/MS we obtained the different fragmentation routes that connect all the ionic populations. In addition, the use of EI-HRMS allowed us to measure the exact masses of all the fragment ions, and, with all this information gathered, we tried to establish a fragmentation scheme concordant with the ascendant and descendant species. CONCLUSIONS: The mass spectrometry studies presented in this work complete our mass studies of Lycopodium alkaloids. The mass spectrometry work presented, has been very useful to confirm the structural determination as well to support as the biogenetic relationships between Lycopodium alkaloids lycodine-type: sauroxine and Ndemethylsauroxine.