Detection of synergistic combinations of Baccharis extracts with Terbinafine against Trichophyton rubrum with high throughput screening synergy assay (HTSS) followed by 3D graphs. Behavior of some of their components

MARÍA VICTORIA RODRIGUEZ; MAXIMILIANO A. SORTINO; JUAN J. IVANCOVICH; JOSÉ M. PELLEGRINO; LAURA S. FAVIER; MARCELA P. RAIMONDI; MARTHA A. GATTUSO; SUSANA A. ZACCHINO

PHYTOMEDICINE

ELSEVIER GMBH

Año: 2013 p. 1230 - 1239

0944-7113

Forty four extracts from nine Baccharis spp. from the Caulopterae section were tested in combination with terbinafine against Trichophyton rubrum with the HTSS assay at six different ratios with the aim of detecting those mixtures that produced a ≥50% statistically significant enhancement of growth inhibition. Since an enhanced effect of a combination respective of its components, does not necessarily indicate synergism, three-dimensional (3D) dose?response surfaces were constructed for each selected pair of extract/antifungal drug with the aid of CombiTool software