The effect of dehydroleucodine in adipocyte differentiation

GALVIS, A. ; MARCANO, A.; STEFANCIN, C.; VILLAVERDE, N.; PRIESTAP, H.A.; TONN, C.E.; LóPEZ, L.A.; BARBIERI, M.A.

EUROPEAN JOURNAL OF PHARMACOLOGY

ELSEVIER SCIENCE BV

Año: 2011 vol. 671 p. 18 - 25

0014-2999

Dehydroleucodine (DhL) is a sesquiterpene lactone of the guaianolide groupwith gastric cytoprotective activity.Recent studies have also demonstrated that DhL inhibits the proliferation of vascular smooth muscle cells. In this study we examined the effect of DhL in the differentiation of 3T3-L1 preadipocytes. The addition of DhL significantly inhibited the differentiation of 3T3-L1 preadipocytes alongwith a significant decrease in the accumulation of lipid content by a dramatic downregulation of the expression of adipogenic-specific transcriptional factors PPARã and C-EBPá. However, phosphorylation of AMPKá, Erk1/2 and Akt1 was not inhibited by DhL treatment. Interestingly, we also found that 11,13-dihydrodehydroleucodine, a derivative of DhL with inactivated á-methylene-ã-lactone function, also inhibited the differentiation of 3T3-L1 preadipocytes. Taken together, these data suggest that DhL has an important inhibitory effect in cellular pathways regulating adipocyte differentiation by modulating the PPARã expression, which is known to play a pivotal role during adipogenesis.