IMASL   20939
INSTITUTO DE MATEMATICA APLICADA DE SAN LUIS "PROF. EZIO MARCHI"
Unidad Ejecutora - UE
artículos
Título:
Determination of hydrodynamic properties of BSA
Autor/es:
R. CURVALE; J.C. CESCO; N. DEBATISTA; N. PAPPANO
Revista:
The Chemical Educator
Editorial:
TCE
Referencias:
Año: 2010 vol. 15 p. 417 - 419
ISSN:
1430-4171
Resumen:
This work allows combining theoretical fundamentals along with the development of abilities and skills on applied hydrodynamics. This is a complementary tool used to establish or to confirm the structures and interactions of biomolecules, especially in diluted solutions. In this laboratory, viscosity measurements of bovine serum albumin solutions at different pH values are used to obtain parameters related to form and size of BSA conformers. This practice, which can be performed in three hours, presents the advantage of using low cost reagents and equipment common to many laboratories, which in turn facilitates student participation in their process of learning.