IMASL   20939
INSTITUTO DE MATEMATICA APLICADA DE SAN LUIS "PROF. EZIO MARCHI"
Unidad Ejecutora - UE
artículos
Título:
Insight into the Molecular Properties of Chitlac, a Chitosan Derivative for Tissue Engineering
Autor/es:
NICOLA DAMELIO, CARMEN ESTEBAN, ANNA COSLOVI, LUIGI FERUGLIO, FULVIO UGGERI, MYRIAM VILLEGAS, JULIO BENEGAS, SERGIO PAOLETTI AND IVAN DONATI
Revista:
JOURNAL OF PHYSICAL CHEMISTRY B - (Print)
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: Washington; Año: 2013 vol. 117 p. 13578 - 13587
ISSN:
1520-6106
Resumen:
ABSTRACT: Chitlac is a biocompatible modified polysaccharide composed of achitosan backbone to which lactitol moieties have been chemically inserted via areductive N-alkylation reaction with lactose. The physical−chemical and biologicalproperties of Chitlac that have been already reported in the literature suggest a highaccessibility of terminal galactose in the lactitol side chain. This finding may accountfor its biocompatibility which makes it extremely interesting for the production ofbiomaterials. The average structure and the dynamics of the side chains of Chitlachave been studied by means of NMR (nuclear Overhauser effect and nuclearrelaxation) and molecular dynamics to ascertain this hypothesis. A completeassignment of the 1H and 13C NMR signals of the modified polysaccharide has beenaccomplished together with the determination of the apparent pKa values of theprimary and secondary amines (6.69 and 5.87, respectively). NMR and MD indicateda high mobility of Chitlac side chains with comparable average internuclear distancesbetween the two techniques. It was found that the highly flexible lactitol side chain in Chitlac can adopt two distinctconformations differing in the orientation with respect to the polysaccharide chain: a folded conformation, with the galactose ringparallel to the main chain, and an extended conformation, where the lactitol points away from the chitosan backbone. In bothcases, the side chain resulted to be highly hydrated and fully immersed in the solvent.