IMIBIO-SL   20937
INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIONES BIOLOGICAS DE SAN LUIS
Unidad Ejecutora - UE
artículos
Título:
Age-Related Changes in Ang II Receptor Localization and Expression in the Developing Auditory Pathway
Autor/es:
ARCE M. ELENA; CIUFFO MARIA GLADYS; SANCHEZ SUSANA INES; CORREA MILAGROS; ARCE M. ELENA; CIUFFO MARIA GLADYS; CORREA MILAGROS; SANCHEZ SUSANA INES
Revista:
NEUROCHEMICAL RESEARCH
Editorial:
SPRINGER/PLENUM PUBLISHERS
Referencias:
Lugar: New York; Año: 2019 vol. 44 p. 412 - 420
ISSN:
0364-3190
Resumen:
We studied Ang II receptor localization in different nuclei of the auditory system, by means of binding autoradiography,during brain development. The inferior colliculus (IC), a large midbrain structure which serves as an obligatory synapticstation in both the ascending and descending auditory pathways, exhibited high Ang II AT2binding at all ages (P0, P8,P15, P30), being maximal at P15. These observations were confirmed by in situ hybridization and immunofluorescence atP15, demonstrating that AT2receptor mRNA localized at the same area recognized by AT2antibodies and anti β III?tubulinsuggesting the neuronal nature of the reactive cells. Ang II AT1receptors were absent at early developmental ages (P0) inall nuclei of the auditory system and a low level was observed in the IC at the age P8. AT2receptors were present at ventralcochlear nucleus and superior olivary complex, being higher at P15 and P8, respectively. We also explored the effect ofprenatal administration of Ang II or PD123319 (AT2 antagonist) on binding of Ang II receptors at P0, P8, P15. Both treatmentsincreased significantly the level of AT2receptors at P0 and P8 in the IC. Although total binding in the whole IC fromP15 animals showed no difference between treatments, the central nucleus of the IC exhibited higher binding. Our resultssupports a correlation between the timing of the higher expression of Ang II AT2receptors in different nuclei, the onset ofaudition and the establishment of neuronal circuits of the auditory pathway.