TRAPPING OF DNA RADICALS WITH THE NITRONE SPIN TRAP 5,5-DIMETHYL-1-PYRROLINE N-OXIDE AND GENOTOXIC DAMAGE: RECENT ADVANCES USING IMMUNO-SPIN TRAPPING TECHNOLOGY.

SANDRA E. GOMEZ MEJIBA; DARIO C RAMIREZ

Mutation Research - Reviews

ELSEVIER SCIENCE BV

Año: 2019

1383-5742

Immuno-spin trapping detection of DNA radicals with the nitrone spin trap 5,5-dimethyl-1-pyrrloine N-oxide (DMPO)has made important contributions towards the understanding of DNA radicalization and genotoxicity at sites ofinflammation. At sites of inflammation, one-electron oxidants and chloramines decay induce oxidation of genomicDNA, genotoxicity and cell transformation. Radicalization of DNA can result in either single- or double-strand breaks,or end-oxidation products at the sugar or bases. If not repaired, these modifications can lead to mutations and celltransformation. If trapped with DMPO, DNA-centered radical decay and subsequent formation of end-oxidationproducts are blocked. Herein we discuss recent literature regarding the use of immuno-spin trapping with DMPO tostudy DNA-centered radicals and their involvement in genotoxicity. This technique has shown the critical role of DNAradicalization in 8-oxo-dG formation and DNA strand breaks in isolated DNA, cells and in whole animals. Combinationof technologies, including immuno-spin trapping and powerful chromatographic and sequencing techniques areneeded to move forward the field towards the detection of specific genes that are susceptible to oxidative damage incells located at sites of inflammation. This is important in order to provide novel information about genotoxicitymechanisms, as well as therapeutic possibilities of DMPO or its derivatives for preventing DNA-centered radicalmediatedcarcinogenesis.