Plant tissue cultures as sources of new ene- and ketoreductase activities

MASCOTTI, ML; ORDEN, AA; CECATI, FM; AGOSTINI, E; MAGALLANES NOGUERA, C; RETA, GF; KURINA-SANZ, M

JOURNAL OF BIOTECHNOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2017 p. 14 - 20

0168-1656

While many redox enzymes are nowadays available  for synthetic applications, the toolbox of ene-reductases is still limited.  Consequently,  the screening  for these enzymes from diverse sources  in the search of new biocatalyst  suitable for green chemistry approaches  is needed. Among 13 plant tissue cultures, Medicago  sativa  and  Tessaria  absinthioides  calli, as well as Capsicum annuum  hairy roots, were selected due to their ability to hydrogenate the C=C double bond of the model substrate 2-cyclohexene-1-one. The three  axenic plant  cultures showed more preference toward highly activated  molecules  such as  nitrostyrene  and maleimide rather than the  classical substrates of the well-known  Old Yellow Enzymes, resembling the skills of the NAD(P)H-dependent flavin-independent enzymes. When the three biocatalytic systems were applied in the reduction of chalcones, T. absinthioides showed high chemoselectivity toward the C=C double bond whereas  the other two demonstrated abilities to biohydrogenate the C=C double bounds and the carbonyl groups in a sequential fashion.