Participation of the extrinsic cholinergic innervation in the

LAURA D. MORALES; ANA C. ANZULOVICH; SANDRA S. VALLCANERAS; SILVIA M. DELGADO; MARÍA B DELSOUC; DANIELA BRONZI; MARILINA CASAIS

GENERAL AND COMPARATIVE ENDOCRINOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2016

0016-6480

Abstract An ex-vivo Coeliac Ganglion-Superior Ovarian Nerve-Ovary (CG-SON-O) system and an ovary without peripheral neural influence from virgin rats in the first proestrous were usedto test whether ovarian extrinsic innervation and nitric oxide (NO) affects steroidogenesis in the ovary. The CG and the ovary were placed in separate buffered-compartments,connected by the SON. Stimulation of the CG was achieved by 10-6  M acetylcholine (Ach).The ovary without peripheral neural influence was placed alone in a bufferedcompartment. To test a possible role of NO in the ovarian response to peripheral neuralinfluence, 100 ìM sodium nitroprusside (SNP, an NO donor) and 100 ìM NG -nitro-Larginine methyl ester (L-NAME, an inhibitor of NO synthase) were added to the ovariancompartment separately. In the CG-SON-O system, SNP into the ovarian compartment increased the concentration of NO, reduced the release of progesterone and increased the release of estradiol (E2), increasing the mRNAs related to their synthesis enzyme. The addition of L-NAME to the ovarian compartment caused an opposite effect. In the ovary alone, NO manifested an antisteroidogenic effect on both hormones. These results showthat the ovarian extrinsic innervation maintains a direct relationship between NO and E2,both needed at high levels during the follicular phase, allowing the continuity of the estrous cycle.