IMIBIO-SL   20937
INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIONES BIOLOGICAS DE SAN LUIS
Unidad Ejecutora - UE
artículos
Título:
Neuromodulation of the luteal regression: presence of progesterone receptors in coeliac ganglion
Autor/es:
GHERSA F; BURDISSO J; VALLCANERAS S; FUENTES F; DE LA VEGA M; DELGADO SM; TELLERIA C; CASAIS M
Revista:
EXPERIMENTAL PHYSIOLOGY.
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2015
ISSN:
0958-0670
Resumen:
The corpus luteum (CL) is a transitory endocrine gland that produces progesterone (P). Atthe end of its useful life, it suffers a process of functional and structural regression untilits complete disappearance from the ovary. To investigate whether P is able to regulate theprocess of luteal regression through the peripheral neural pathway, we used the coeliac ganglion(CG)?superior ovarian nerve?ovary system from rats on day 21 of pregnancy. We stimulatedthe CG with P and analysed the functional regression through ovarian P release measured byradioimmunoassay, expression by RT-PCR and activity of luteal 3β- and 20α-hydroxysteroiddehydrogenase (anabolic and catabolic P enzymes, respectively). The luteal structural regressionwas evaluated through a study of apoptosis measured by TUNEL assay and the expression ofapoptotic factors, such as Bcl-2, Bax, Fas and Fas ligand (FasL) by RT-PCR. To explore whetherthe effects mediated by P on the CL may be associated with P receptors, their presence in theCG was investigated by immunohistochemistry. In the group stimulated with P in the CG,the ovarian P release and the 3β-hydroxysteroid dehydrogenase activity increased, whereas theexpression and activity of 20α-hydroxysteroid dehydrogenase decreased. In addition, a decreasein the number of apoptotic nuclei and a decrease of the expression of FasL were observed.