ORIGINAL

VALLCANERAS S; DE LA VEGA M; DELGADO SM; MOTTA A; TELLERIA C; RASTRILLA AM; CASAIS M

REPRODUCTION FERTILITY AND DEVELOPMENT

CSIRO PUBLISHING

Lugar: Collingwood; Año: 2014

1031-3613

There is considerable evidence of the neuroendocrine control involved in luteal regression in the rat. In addition, circulating prolactin (PRL), which increases during the night before parturition, may gain access to the coeliac ganglion (CG), indirectly impacting the physiology of the ovary because of the known connection between the CG and the ovary via the superior ovarian nerve (SON). In this work we investigated in the CG?SON?ovary system and whether PRL 5 added to the CG has an impact, indirectly via the SON, on luteal regression on Day 21 of pregnancy. The system was incubated without (control) or with PRL added to the CG. We measured the ovarian release of progesterone (P), oestradiol and prostaglandin F2 alpha (PGF2a) by radioimmunoassay, and nitrites (NO) by the Griess method. Luteal mRNA expression of 3b-hydroxysteroid dehydrogenase (3b-HSD), 20a-HSD, aromatase, inducible nitric oxide synthase (iNOS) and apoptosis regulatory factors was analysed by reverse transcription?polymerase chain reaction. P release, the 10 expression of Bcl-2 and the Bcl-2 : Bax ratio was lower than control preparations, while the expression of 20a-HSD and the release of NO and PGF2a were higher in the experimental group. In conclusion, PRL acts at the CG and, by a neural pathway, modulates luteal function at the end of pregnancy.