Inmuno-spin trapping from biochemistry to medicine: Advances, challenges, and pitfalls. Focus on protein-centered radicals

SANDRA E. GOMEZ-MEJIBA, ZILI ZHAI, MARIA C. DELLA-VEDOVA, MARCOS D. MUÑOZA, SAURABH CHATTERJEE, RHEAL A. TOWNER, KENNETH HENSLEY, ROBERT A. FLOYD, RONALD P. MASON, DARIO C. RAMIREZ

ELSEVIER SCIENCE B. V.

Elsiever

Lugar: Amsterdam ; Año: 2014 vol. 1840 p. 722 - 729

Immuno-spin trapping from biochemistry to medicine: Advances,challenges, and pitfalls. Focus on protein-centered radicals Sandra E. Gomez-Mejiba a, Zili Zhai b, Maria C. Della-Vedova a, Marcos D. Muoz a, Saurabh Chatterjee c, Rheal A. Towner d, Kenneth Hensley e, Robert A. Floyd f, Ronald P. Mason g, Dario C. Ramireza,h,*,1 Abstract Inmuno-spin trapping (IST) is based on te reaction of a spin trap with a free radical to form a stable nitrone aduct, followed by the use of antibodies, rather than traditional electron paramagnetic resonance spectroscopy, to detect the nitrone adduc. IST has been successfully applied to mechanistic in vitro studies, and recently, macromolecule-centered radicals have been detected in models of drug induced agranulocytosis, hepatotoxicity, cardiotoxicity, and ischemia/reperfusion, as well as in models of neurological, metabolic and immunological disease. To critically evaluate advances, challenges, and pitfalls as well as the scientific opportunities of IST as applied to the study of protein-centered free radicals generated in stressed organelles, cells, tissues and animal models of disease and exposure