Cadmium-induced oxidative stress and histological damage in the myocardium. Effects of a soy-based diet.

FERRAMOLA ML, PÉREZ DÍAZ MF, HONORÉ SM, SÁNCHEZ SS, ANTÓN RI, ANZULOVICH AC, GIMÉNEZ MS.

TOXICOLOGY AND APPLIED PHARMACOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2012 p. 360 - 369

0041-008X

Toxicol Appl Pharmacol. 2012 Dec 15;265(3):380-9. doi: 10.1016/j.taap.2012.09.009. Epub 2012 Sep 17. Cadmium-induced oxidative stress and histological damage in the myocardium. Effects of a soy-based diet. Ferramola ML, Pérez Díaz MF, Honoré SM, Sánchez SS, Antón RI, Anzulovich AC, Giménez MS. Source Department of Biochemistry and Biological Sciences, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, IMIBIO-SL, CONICET, San Luis, Argentina. Abstract Cd exposure has been associated to an augmented risk for cardiovascular disease. We investigated the effects of 15 and 100 ppm of Cd on redox status as well as histological changes in the rat heart and the putative protective effect of a soy-based diet. Male Wistar rats were separated into 6 groups and treated during 60 days as follows: groups (1), (2) and (3) were fed a casein-based diet; groups (4), (5) and (6), a soy-based diet; (1) and (4) were given tap water; (2) and (5) tap water containing 15 ppm of Cd²⁺; and (3) and (6) tap water containing 100 ppm of Cd²⁺. Serum lipid peroxides increased and PON-1 activity decreased in group (3). Lipoperoxidation also increased in the heart of all intoxicated groups; however protein oxidation only augmented in (3) and reduced glutathione levels diminished in (2) and (3). Catalase activity increased in groups (3) and (6) while superoxide dismutase activity increased only in (6). Glutathione peroxidase activity decreased in groups (3) and (6). Nrf2 expression was higher in groups (3) and (6), and MTI expression augmented in (3). Histological examination of the heart tissue showed the development of hypertrophic and fusion of cardiomyocytes along with foci of myocardial fiber necrosis. The transmission electron microscopy analysis showed profound ultra-structural damages. No protection against tissue degeneration was observed in animals fed the soy-based diet. Our findings indicate that even though the intake of a soy-based diet is capable of ameliorating Cd induced oxidative stress, it failed in preventing cardiac damage. Copyright © 2012 Elsevier Inc. All rights reserved. PMID: 22995158 [PubMed - indexed for MEDLINE]