Inclusion complex of 2-chlorobenzophenone with cyclomaltoheptaose (B-cyclodextrin); temperature, solvent effects and molecular modeling

MATIAS I. SANCHO; ESTELA GASULL; SONIA E. BLANCO; EDUARDO A. CASTRO

CARBOHYDRATE RESEARCH

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2011 vol. 346 p. 1978 - 1984

0008-6215

A thermodynamic study of the inclusion process between 2-chlorobenzophenone (2ClBP) and cyclomaltoheptaose (β-cyclodextrin, β-CD) was performed using UV?vis spectroscopy, reversed-phase liquid chromatography (RP-HPLC), and molecular modeling (PM6). Spectrophotometric measurements in aqueous solutions were performed at different temperatures. The stoichiometry of the complex is 1:1 and its apparent formation constant (Kc) is 3846 M−1 at 30 °C. Temperature dependence of Kc values revealed that both enthalpy (ΔH° = −10.58 kJ/mol) and entropy changes (ΔS° = 33.76 J/K mol) are favorable for the inclusion process in an aqueous medium. Encapsulation was also investigated using RP-HPLC (C18 column) with different mobile-phase compositions, to which β-CD was added. The apparent formation constants in MeOH?H2O (KF) were dependent of the proportion of the mobile phase employed (50:50, 55:45, 60:40 and 65:35, v/v). The KF values were 419 M−1 (50% MeOH) and 166 M−1 (65% MeOH) at 30 °C. The thermodynamic parameters of the complex in an aqueous MeOH medium indicated that this process is largely driven by enthalpy change (ΔH° = −27.25 kJ/mol and ΔS° = −45.12 J/K mol). The results of the study carried out with the PM6 semiempirical method showed that the energetically most favorable structure for the formation of the complex is the ?head up? orientation.