INQUISAL   20936
INSTITUTO DE QUIMICA DE SAN LUIS "DR. ROBERTO ANTONIO OLSINA"
Unidad Ejecutora - UE
artículos
Título:
Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients
Autor/es:
ORTEGA, FRANCISCO G.; DE MIGUEL-PÉREZ, DIEGO; TORTELLA, GONZALO; BERTOTTI, MAURO; RODRÍGUEZ-MARTÍNEZ, ALBA; LORENTE, JOSÉ A.; SAPAG, KARIM; REGIART, MATÍAS D.; SERRANO, MARÍA J.; RUBILAR, OLGA; FERNÁNDEZ-BALDO, MARTÍN A.
Revista:
ANALYTICAL CHEMISTRY
Editorial:
AMER CHEMICAL SOC
Referencias:
Año: 2021 vol. 93 p. 1143 - 1153
ISSN:
0003-2700
Resumen:
This study is focused on identifying novel epithelial markers in circulating extracellular vesicles (EVs) through the development of a dual sandwich-type electrochemical paper-based immunosensor for Claudin 7 and CD81 determination, as well as its validation in breast cancer (BC) patients. This immunosensor allows for rapid, sensitive, and label-free detection of these two relevant BC biomarkers. Under optimum conditions, the limit of detection for Claudin 7 was 0.4 pg mL-1, with a wide linear range of 2 to 1000 pg mL-1, while for CD81, the limit of detection was 3 pg mL-1, with a wide linear range of 0.01 to 10 ng mL-1. Finally, we validated Claudin 7 and CD81 determination in EVs from 60 BC patients and 20 healthy volunteers, reporting higher diagnostic accuracy than the one observed with classical diagnostic markers. This analysis provides a low-cost, specific, versatile, and user-friendly strategy as a robust and reliable tool for early BC diagnosis.