INQUISAL   20936
INSTITUTO DE QUIMICA DE SAN LUIS "DR. ROBERTO ANTONIO OLSINA"
Unidad Ejecutora - UE
artículos
Título:
Food safety control of zeranol through voltammetric immunosensing on Au?Pt bimetallic nanoparticle surfaces
Autor/es:
DANIEL MATÍAS G. REGIART; SIRLEY V. PEREIRA; VIVIANA G. SPOTORNO; FRANCO A. BERTOLINO; JULIO RABA
Revista:
ANALYST
Editorial:
ROYAL SOC CHEMISTRY
Referencias:
Lugar: CAMBRIDGE; Año: 2014 vol. 139 p. 4702 - 4709
ISSN:
0003-2654
Resumen:
This study reports an accurate and sensitive strategy for zeranol (ZER) determination in bovine urine samples. ZER is a mycotoxin widely used as a synthetic growth promoter in the livestock production whose residues could present a potential risk for human health. Therefore, its use as an animal feed additive has been banned in most countries. ZER determination was accomplished using an electrochemical system in which bimetallic Au?Pt nanoparticles (Au?PtNPs) were electro-synthesized on a screen-printed carbon electrode (SPCE). The obtained Au?PtNP platform was immunofunctionalized using specificanti-ZERantibodiesasa strategy to avoid potential interference. After biorecognition, ZER was directly oxidized and detected by square-wave voltammetry (SWV). The Au?PtNP surface was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and cyclic voltammetry (CV). The limit of detection calculated was 0.01 ng mL1 with a wide linear range from 0.03 to 30 ng mL1 . This method promises to be suitable for ZER quantification in bovine urine samples ensuring food quality and safety, as well as consumer?shealth.