INQUISAL   20936
INSTITUTO DE QUIMICA DE SAN LUIS "DR. ROBERTO ANTONIO OLSINA"
Unidad Ejecutora - UE
artículos
Título:
Single-step procedure for trace element determination in synovial fluid by dynamic reaction cell-inductively coupled plasma mass spectrometry
Autor/es:
MARIO MOYANO; ERNESTO VERNI; HÉCTOR TAMASHIRO; SILVIA DIGENARO; LUIS D. MARTINEZ; GIL RAÚL A.
Revista:
MICROCHEMICAL JOURNAL
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 112 p. 17 - 24
ISSN:
0026-265X
Resumen:
A fast and single-step procedure for the dissolution of human synovial fluid in formic acid and further determination by dynamic reaction cell-inductively coupled plasma mass spectrometry (DRC-ICPMS) with a high-efficiency sample introduction system was developed. The samples were collected, treated and analyzed in the same screwcapped tubes. In order to overcome the effect of considerable carbon content, the sample introduction, nebulization and ICP operating conditions were carefully optimized. Furthermore, DRC technology with CH4 as reaction gas was used for the elimination of spectral interferences due to polyatomic ions. The effect of the sample matrix was evaluated and mitigated through comparison of direct calibration against aqueous standards, direct calibration in formic acid media and analyte addition calibration. The recommended procedure involved low dilution and low detection limits (from 0.003 μg L−1 for U to 13.3 μg L−1 for Ti) with adequate precision (from 0.6% for Co to 6.6% for Ti). The proposed method was successfully applied to determine 16 trace elements in concentrations from 0.03 μg L−1 (Cd) to 88.2 μg L−1 (Cu) in human synovial fluid samples.