INQUISAL   20936
INSTITUTO DE QUIMICA DE SAN LUIS "DR. ROBERTO ANTONIO OLSINA"
Unidad Ejecutora - UE
artículos
Título:
A NOVEL APPLICATION OF IMMOBILIZATION ON MEMBRANES FOR THE SEPARATION AND SPECTROFLUORIMETRIC QUANTIFICATION OF AMILORIDE AND FUROSEMIDE IN PHARMACEUTICAL SAMPLES
Autor/es:
PERALTA, CECILIA; FERNANDEZ, LILIANA; MASI, ADRIANA
Revista:
ANALYTICA CHIMICA ACTA
Editorial:
Elsevier
Referencias:
Año: 2010 vol. 661 p. 85 - 90
ISSN:
0003-2670
Resumen:
A new, simple and highly sensitive method for spectrofluorimetric determination of amiloride (AMI) and
furosemide (FUR) in pharmaceuticals is presented. The proposed method is based on the separation of
AMI from FUR by solid-phase extraction using a nylon membrane, followed by spectrofluorimetric determination
of both drugs, on the solid surface and the filtered aqueous solution, respectively. AMI shows
low native fluorescence, but its separation-preconcentration by immobilization (solid-phase extraction)
on nylon membrane surface provides a considerable enhancement in fluorescence intensity. The fluorescence
determination is carried out at ex = 237, em = 415nmfor FUR; and ex = 365, em = 406nmfor AMI.
The calibration graphs are linear in the range 3.20×10−4 to 0.8gmL−1and 1.33×10−3 to 4.0gmL−1,
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
The calibration graphs are linear in the range 3.20×10−4 to 0.8gmL−1and 1.33×10−3 to 4.0gmL−1,
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
The calibration graphs are linear in the range 3.20×10−4 to 0.8gmL−1and 1.33×10−3 to 4.0gmL−1,
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
ex = 237, em = 415nmfor FUR; and ex = 365, em = 406nmfor AMI.
The calibration graphs are linear in the range 3.20×10−4 to 0.8gmL−1and 1.33×10−3 to 4.0gmL−1,
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
×10−4 to 0.8gmL−1and 1.33×10−3 to 4.0gmL−1,
for AMI and FUR, respectively, with a detection limit of 9.62×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations
×10−5 and 4.01×10−4 gmL−1 (S/N= 3). The
commonly found excipients in commercial pharmaceutical formulations do not interfere. The developed
method is successfully applied to the determination of both drugs in pharmaceutical formulations