JEREZ Susana Josefina
congresos y reuniones científicas
INFLUENCE OF DIET ON RABBIT PLASMATIC LIPIDS
MIRTA MEDINA; SILVIA SAAD DE SCHOOS; CARINA VANNIEUWENHOVE; MARÍA PERAL DE BRUNO; SUSANA JEREZ
Tafi del Valle-Tucuman
Congreso; XXV Jornadas de la Asociacion de Biologia de Tucuman; 2009
Asociacion de Biologia de Tucuman
Cholesterol and fatty acid intake influence on cardiovascular diseases development. The aim of this work was to determine the influence of higher cholesterol and fat diet on rabbit plasmatic lipids. Flander rabbit were separated in 3 groups fed with: commercial diet (Control), commercial diet supplemented with 1% cholesterol (DH), commercial diet supplemented with 10 % of fat (DG). Blood was collected from carotid artery and plasma was obtained for triglycerides (TG) and cholesterol (CT, LDL, HDL) analysis (kits) and fatty acid by gaseous chromatography. Higher CT and LDL levels were determined in DH than control group (5.24 vs 0.55 and 2.86 vs. 0.22g/l, respectively). Atherogenic index (CT/HDL) was higher in DH (11) than Control (1.75) and DG (1.5) group. Higher palmitoleic (1.0 vs 3.0 %), linolenic (1.0 vs 1.8 %) and araquidonic (3.0 vs 6.3 %) acid content were observed in Control than DH group. DG group varied from Control in araquidonic (5.6 vs. 3.0), eicosapentanoic (EPA) (0.17 vs 0.24 %) and docosahexanoic (DHA) (3.7 vs. 1.97 %). Ä6 desaturase index was higher in DH (0.25) than DG (0.23) and Control (0.11) rabbits. This index indicates an increase of enzymatic activity that convert linoleic acid in araquidonic acid. DHA increase and EPA decrease of DG group may be related to vascular regulation. Conclusion: diet rich in cholesterol or fat modified plasmatic lipids and the model presented may be useful for other determinations.